Journal: EMBO Reports
Article Title: miR-7 controls glutamatergic transmission and neuronal connectivity in a Cdr1as-dependent manner
doi: 10.1038/s44319-024-00168-9
Figure Lengend Snippet: ( A ) Histogram and scatter plot show distribution of number of active synapses of WT neurons cultured in media with 2 mM Ca +2 to ensure neuronal firing. AP-evoked glutamate release calculated as evoked probability (left) and spontaneous glutamate release calculated as spontaneous frequency (right), respectively. ( B ) Histogram and scatter plot show distribution of number of active synapses of Cdr1as-KO neurons cultured in media with 2 mM Ca +2 to ensure neuronal firing. AP-evoked glutamate release calculated as evoked probability (left) and spontaneous glutamate release calculated as spontaneous frequency (right), respectively. ( C ) Scheme of the Multi-electrode Array recording protocol (Axion Biosystems, CytoView MEA 48, “Methods”). Second panel: representative image of cultured neurons DIV14 in a recording well. white: electrodes, red: mCherry reporter. Third panel: schematic representation of output data, extracellular field potentials and neuronal network activity. AP: Adaptative threshold 6 SD. Sampling frequency 12.5 kHz. Active electrode selection criteria 5 spikes/minute. ( D ) Mean Firing Rate: Total number of spikes per single electrode divided by the duration of the analysis (600 s), in Hz. Each column represents recordings from a single biological replicate. Each dot represents recordings from a single electrode. ( E ) Example raw spikes from multi electrodes Array recording: 100 ms raw spikes of WT and Cdr1as-KO neurons DIV21. Each row represents one independent electrode (black bars).
Article Snippet: Cells were seeded in 48-well CytoView MEA plates with 16 poly-3,4-ethylendioxythiophen (PEDOT) electrodes per well (Axion Biosystems).
Techniques: Cell Culture, Activity Assay, Sampling, Selection